You have no items in your shopping cart.
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL. |
| Target | TNF-alpha |
| Tag | His Tag |
| MW | 18.2 kDa |
| Purity | > 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Val 77 - Leu 233 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−TNFR2 Recombinant Rabbit Monoclonal Antibody [orb612211]
FC, ICC, IF, IHC-Fr, IHC-P, WB
Rat
Human, Mouse
Rabbit
Recombinant
Unconjugated
50 μl, 100 μlRecombinant Human Tumor necrosis factor (TNF) Protein [orb624131]
Greater than 90% as determined by SDS-PAGE.
19.4 kDa
Yeast
1 mg, 20 μg, 100 μgHuman TNF protein [orb705324]
Greater than 85% as determined by SDS-PAGE.
19.4 kDa
E.coli
20 μg, 100 μg, 1 mgHuman TNF protein [orb594840]
Greater than 95% as determined by SDS-PAGE.
17.5 kDa
E.coli
500 μg, 1 mg, 50 μg, 10 μgHuman TNF protein [orb594841]
Greater than 95% as determined by SDS-PAGE.
18.5 kDa
E.coli
50 μg, 500 μg, 1 mg, 10 μg

Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immobilized Human TNF-alpha, His Tag at 0.2 ug/ml (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.
Quick Database Links
NCBI Reference Sequences
−| Protein | NP_000585.2 |
|---|
Documents Download
Request a Document
Protocol Information
TNF-alpha Recombinant Protein (orb1227719)
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a Review












