TNF-alpha (Tumor Necrosis Factor alpha) Antibody

SKU: orb2317803

Description

TNF-alpha (Tumor Necrosis Factor alpha) Antibody

Research Area

,AKT Signaling|Apoptosis|Autophagy|Cardiovascular|Colon Cancer|Cytokine Signaling|Developmental Biology|Hematopoietic Stem Cells|Immunology|MAPK Signaling|Neuroinflammation|Ovarian Cancer|Signal Transduction

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human, Rat
• Application Notes:
  Positive Control: HeLa, HL-60 or A431 cells. Pancreas or Histiocytoma.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant full-length human TNF-alpha protein
• Target: TNF
• Molecular Weight: 17kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

NCBI Gene ID

7124