TNF-alpha (Tumor Necrosis Factor alpha) Antibody

SKU: orb2319979

Description

TNF-alpha (Tumor Necrosis Factor alpha) Antibody

Research Area

,AKT Signaling|Apoptosis|Autophagy|Cardiovascular|Colon Cancer|Cytokine Signaling|Developmental Biology|Hematopoietic Stem Cells|Immunology|MAPK Signaling|Neuroinflammation|Ovarian Cancer|Signal Transduction

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Inhibits Tumor Growth (Order Ab without BSA/Azide); Neutralizes rTNF- Mediated Cytotoxicity (Order Ab without BSA/Azide); Protects Mice Against Toxicity of rhTNF- (Order Ab without BSA/Azide)Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: J2D10
• Immunogen: Recombinant human TNF-alpha
• Target: TNF
• Source/Expression System: CHO cells
• Molecular Weight: 17kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

NCBI Gene ID

7124