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TNF-beta Antibody

SKU: orb1671469

Description

TNF-beta Antibody

Research Area

,Apoptosis,Obesity,Neuroscience,Cell Cycle,Stem Cell,Cancer,Immunology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TNF-beta as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-beta. Western Blot To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-beta. Anti-Human TNF-beta specific antibody was purified by affinity chromatography employing immobilized Human TNF-beta matrix.
TargetLTA
PurificationTNF-beta specific antibody was purified by affinity chromatography employing immobilized Human TNF-beta matrix
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Tumor Necrosis Factor-beta, TNFSF1B, Lymphotoxin-alpha (LT-alpha)

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP01374

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.05 mg
$ 720.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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