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Description
Research Area
,Apoptosis,Cancer,Cell Cycle,Chemokines & Cytokines,Immunology,Signal Transduction,Other
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | TNF-R1:Fc fusion protein blocks TNF-alpha-mediated biological effect with an inhibitory activity in a concentration range of 0.1 to 1ug/ml. |
| Target | TNFRSF1A |
| Tag | Fc Tag |
| Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of human TNF-R1 (aa 22-211) is fused to the Fc portion of human IgG1. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 50 μl sterile water. |
| Concentration | 1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−p55, CD120a, TNFRSF 1A, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNF Receptor 1
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Human TNFRSF1A Protein, hFc Tag [orb1291011]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46.6 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1A-hFc is approximately 55-70 kDa due to glycosylation.
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Greater than 85% as determined by SDS-PAGE.
22.7 kDa
Yeast
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Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman TNFRSF1A protein [orb392596]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
E. coli
500 μg, 50 μg, 10 μgMouse TNFRSF1A protein [orb392597]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
E. coli
500 μg, 50 μg, 10 μg
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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