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Description
Research Area
,Apoptosis,Cancer,Cell Cycle,Chemokines & Cytokines,Immunology,Signal Transduction,Other,Inflammasomes
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNFR1/CD120a Protein,C-Fc Tag at 5 μg/mL (100 μl/well) can bind Biotinylated Human TNF-alpha Protein . The EC50 is typically 2. 8-11. 4 ng/mL. |
| Target | TNFRSF1A |
| Tag | C-Fc Tag |
| MW | 47.9 kDa |
| Purity | > 98% as determined by SDS-PAGE. |
| Protein Sequence | Ile 22 - Thr 211 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60
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The purity of rh TNFR1 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
TNFR1 Recombinant Protein (orb1227857)
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