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Description
Research Area
,Apoptosis,Chemokines & Cytokines,Growth Factors,Immunology,Other
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human TNFa at 5μg/mL (100μl/well) can bind human TNFR2. The EC50 of human TNFR2 is 647 ng/mL. |
| Target | TNFRSF1B |
| Tag | His Tag |
| MW | 26 kDa |
| Purity | > 95% as determined by SDS-PAGE. |
| Protein Sequence | Leu 23 - Asp 257 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 26.2 kDa after removal of the signal peptide. The apparent molecular mass of mTNFRSF1B-His is approximately 25-55 kDa due to glycosylation.
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20 μg, 1 mg, 5 μgHuman TNF Receptor II protein [orb754265]
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Human TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
TNFRII Recombinant Protein (orb1227855)
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