TRAIL Antibody [1.1_1A7-2B7]

SKU: orb1272251

Description

TRAIL Antibody [1.1_1A7-2B7]

Research Area

,Apoptosis,Cancer

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot:To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Clone No.: 1.1_1A7-2B7
• Immunogen: The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTRAIL/APO-II as the immunizing antigen.
• Target: TNFSF10
• Purification: This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APO2L, Tumor Necrosis Factor (ligand) Superfamily Member 10, CD253, APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2

Quick Database Links

Gene Symbol

TNFSF10

UniProt

P50591

RefSeq:P50591