Transferrin Mouse Monoclonal Antibody (Serum Loading Control)

SKU: orb704517

Description

Transferrin Mouse Monoclonal Antibody (Serum Loading Control)

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:5000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Reactivity: Bovine, Human, Mouse, Rat
• Predicted Reactivity: Bovine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 12A7
• Immunogen: Full length native Transferrin protein purified from human plasma
• Target: TF
• Molecular Weight: 77 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apotransferrin; TRF; eta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; TRFE_HUMAN; beta-1 metal-binding globulin; Serotransferrin.

25 ug total protein per lane of various lysates probed with Transferrin monoclonal antibody, unconjugated (orb704517) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Blank control (black line): HepG2. Primary Antibody (green line): Mosue Anti-Transferrin (Serum Loading Control) antibody (orb704517), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (orb704517) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (orb704517) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

TF

UniProt

P02787