TRAP Antibody (N-term)

SKU: orb1429780

Description

Rabbit polyclonal antibody to TRAP.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics,Primary Antibodies;Cardiovascular;Immunology;T cell receptor signaling;Cytokine;Allograft rejection;Intestinal immune network for IgA production

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19150
• Immunogen: Synthetic Peptide
• Target: CD40LG
• Molecular Weight: 29274
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP

All lanes: Anti-TRAP Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: THP-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of TRAP Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TRAP antibody (N-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TRAP Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of TRAP antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). TRAP (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

CD40LG

UniProt

P29965

RefSeq (Protein):NP_000065.1