TRIM27 Antibody

SKU: orb2636837

Description

Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells.

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the TRIM27 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: PCRP-TRIM27-1B3
• Immunogen: Recombinant full-length human TRIM27 protein was used as the immunogen for the TRIM27 antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This TRIM27 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed U87 cells using TRIM27 antibody (green, clone PCRP-TRIM27-1B3) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with TRIM27 antibody (blue, clone PCRP-TRIM27-1B3), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using TRIM27 antibody (clone PCRP-TRIM27-1B3). These results demonstrate the foremost specificity of the PCRP-TRIM27-1B3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TRIM27 antibody (PCRP-TRIM27-1B3) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14373