Trk B (phospho Tyr516) rabbit pAb

SKU: orb769304

Description

Rabbit polyclonal antibody to Trk B (phospho-Tyr516)

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr515. AA range:481-530
• Molecular Weight: 145
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-NTRK2 antibody, anti-TRKB antibody, anti-BDNF/NT-3 growth factors receptor antibody, anti-GP145-TrkB antibody, anti-Trk-B antibody, anti-Neurotrophic tyrosine kinase receptor type 2 antibody, anti-TrkB tyrosine kinase antibody, anti-Tropomyosin-related kinase B antibody

Western Blot analysis of RAT-MUSCLE cells using Phospho-Trk B (Y516) Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B (Phospho-Tyr515) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.