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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:2000-1:4000 Immunohistochemistry (Paraffin) 1:200-1:400 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | Troponin T, cardiac |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-Troponin T, cardiac muscle antibody - at 1/3000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294292 overnight at 4°C. Troponin I, cardiac muscle (orb1294291).

All lanes: Anti-Troponin T, cardiac muscle antibody - at 1/3000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294292 overnight at 4°C.
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Troponin T, cardiac (orb1294292)
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