TUBA1C Antibody (C-term)

SKU: orb1937770

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Antibody Collections;Signal Transduction;Loading Control

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Other

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB31971
• Target: This TUBA1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-441 amino acids from the C-terminal region of human TUBA1C.
• Molecular Weight: 49895 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain, TUBA1C, TUBA6

Anti-TUBA1C Antibody (C-term) at 1:2000 dilution + A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining TUBA1C in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

TUBA1C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TUBA1C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from mouse liver and rat testis tissue lysate (from left to right), using TUBA1C Antibody (C-term). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

This TUBA1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-441 amino acids from the C-terminal region of human TUBA1C.

UniProt

Q9BQE3

RefSeq (Protein):NP_116093.1