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−Item 1 of 8
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 2A1A9 |
| Immunogen | Purified recombinant fragment of human TUBB1 (AA: 33-166) expressed in E. Coli. |
| Target | TUBB1 |
| Molecular Weight | 50.3kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tubulin beta-1 chain
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of HEK293 using TUBB1 antibody
Western blot analysis of K562 (Lane1), HepG2 (Lane2), A431 (Lane3), Jurkat (Lane4), Hela (Lane5), NIH/3T3 (Lane6), Cos7 (Lane7) and PC12 (Lane8) cell lysate using TUBB1 antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of A431 cells using TUBB1 antibody
Immunofluorescence analysis of Hela cells using TUBB1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using TUBB1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using TUBB1 antibody
Western blot analysis of human recombinant protein using TUBB1 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
TUBB1 (orb229789)
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