Turbo Nuclease

SKU: orb532709

Description

Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single-or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner. Endonuclease from Serratia macescens recombinant expressed and purified from E.coli. Catalytic activity from pH 6 to 10 (optimal around 8.8) and tem-perature 0 to 44 °C. High catalytic efficiency (34-fold greater than DNase I). Activity requires the presence of Mg2+ (optimum 2 mM).

Images & Validation

• Application Notes:
  Turbo Nuclease is very effective in degrading nucleic acid from pro-tein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required: Reduction of viscosity from cell lysates. Prevention of cell clumping. Elimination of unspecific protein-DNA complexes prior 2D-or native gel electrophoresis. Removal of nucleic acids from large-scale protein preparations.

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Key Properties

• Source: E. coli
• Biological Origin: Serratia marcescens

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl 2, 1 mM DTT and 50% [v/v] glycerol)
• Concentration: 250 units/µl
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only