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−Item 1 of 7
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 10E4E6 |
| Immunogen | Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli. |
| Target | TWIST1 |
| Molecular Weight | 21kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
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Western blot analysis of human TWIST1 using TWIST1 antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of TWIST1 antibody
Flow cytometric analysis of Hela cells lysates using TWIST1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using TWIST1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using TWIST1 antibody
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using TWIST1 antibody
Western blot analysis of NIH/3T3 (Lane 1), JURKAT (Lane 2), HELA (Lane 3), A549 (Lane 4), RAJI (Lane 5) OCM-1 (Lane 6) cell lysate using TWIST1 antibody
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
TWIST1 (orb153461)
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