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UBC9 Antibody
SKU: orb166944
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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Primary Antibodies;Cell Biology;Metabolism;Viral process;Host-virus interaction
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution Range | WB: 1:100-500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 67AT1273.95.90 |
| Target | UBE2I |
| Molecular Weight | 18007 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti UBE2I antibody, anti UBC9 antibody, anti UBCE9 antibody, anti SUMO-conjugating enzyme UBC9 antibody, anti SUMO-protein ligase antibody, anti Ubiquitin carrier protein 9 antibody, anti Ubiquitin carrier protein I antibody, anti Ubiquitin-conjugating enzyme E2 I antibody, anti Ubiquitin-protein ligase I antibody, anti p18 antibody
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All lanes: Anti-UBC9 Antibody at 1:1000-1:2000 dilution. Lane 1: human heart lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-UBC9 Antibody in Ramos cell line lysates (35 μg/lane). UBC9 (arrow) was detected using the purified Mab.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_919237.1, NP_003336.1, NP_919235.1, NP_919236.1 |
|---|
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Datasheet
Product Information
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Maarifi, Ghizlane et al. Differential effects of SUMO1 and SUMO3 on PKR activation and stability Sci Rep, 8, 1277 (2018)
UBC9 Antibody (orb166944)
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