UBC9 (UBE2I) Antibody (N-term)

SKU: orb1938824

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cell Biology;Metabolism;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB2811
• Target: This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).
• Molecular Weight: 18007 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-UBE2I Antibody (N-term) in A2058 cell line lysates (35 ug/lane). UBE2I (arrow) was detected using the purified Pab.

Western blot analysis of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.

Quick Database Links

Gene Symbol

This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).

UniProt

P63279

RefSeq (Protein):NP_919237.1, NP_003336.1, NP_919235.1, NP_919236.1