Ubiquitin (Autophagy Marker) Antibody

SKU: orb2316091

Description

Ubiquitin (Autophagy Marker) Antibody

Research Area

,Apoptosis|Autophagy|Cardiovascular|Cytokine Signaling|Developmental Biology|Hypoxia|Immunology|Infectious Disease|Neuroscience|Ovarian Cancer|Signal Transduction|Transcription Factors

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c
• Clone No.: UBB/1748
• Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
• Target: IGKV1D-16
• Source/Expression System: HEK293 cells
• Molecular Weight: 9kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epididymis secretory protein Li 50; Polyubiquitin B; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2

Quick Database Links

Gene Symbol

IGKV1D-16

UniProt

P62987, P62988, P62979

NCBI Gene ID

7314