VEGFR1 Rabbit Polyclonal Antibody

SKU: orb11556

Description

VEGFR1 Rabbit Polyclonal Antibody

Research Area

Angiogenesis, Cancer Research, Cancer Research, Cardiovascular Research, Cell Markers, Cardiovascular Research, Growth Factors, Growth Factors, Cancer Research, Hypoxia, Tyrosine Kinases, Cell Biology, VEGF Receptors, Vascular

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse VEGFR1 (1162-1260/1333aa)
• Target: FLT1
• Molecular Weight: 147 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 1; VEGF-R1; VEGFR-1; VEGF Receptor 1; FLT-1; vascular permeability factor receptor; vascular endothelial growth factor receptor; vascular endothelial growth factor receptor-1; fms-related tyrosine kinase 1; vascular endothelial growth factor/vascular permeability factor receptor; AI323757; FLT; FLT1; sFlt1; VGFR1_HUMAN; VGFR1_MOUSE.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (orb11556) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293), Primary: Anti-VEGFR1 (orb11556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 147 kDa, Observed band size: 140 kDa.

Tissue/Cell: 293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (VEGFR1) polyclonal Antibody, Unconjugated (orb11556) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

FLT1

Graham, N. et al. Placental expression of anti-angiogenic proteins in mirror syndrome: a case report Placenta, 33, 528-531 (2012)

PubMed: 22401877

Applications

IHC

Reactivity

Human