VEGFR2 (FLK1/KDR) Antibody (C-term)

SKU: orb38298

Description

Rabbit polyclonal antibody to VEGFR2

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Epigenetics,Developmental Biology,Infectious Diseases,Primary Antibodies;Cancer;Cardiovascular;Immunology;Metabolism;Microbiology;Signal Transduction;Anti-Kinome Antibodies;Viral process;Host-virus interaction;Immune system process

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:10-50, FACS: 1:10-50, IHC-P: 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB01536
• Immunogen: Synthetic Peptide
• Target: KDR
• Molecular Weight: 151527
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD309 antibody, anti CD309 antigen antibody, anti Fetal liver kinase 1 antibody, anti FLK-1 antibody, anti FLK1 antibody, anti FLK1, mouse, homolog of antibody, anti KDR antibody, anti Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody, anti Kinase insert domain receptor antibody, anti KRD1 antibody, anti Ly73 antibody, anti Protein tyrosine kinase receptor FLK1 antibody, anti Protein-tyrosine kinase receptor flk-1 antibody, anti soluble VEGFR2 antibody, anti Tyrosine kinase growth factor receptor antibody, anti Vascular endothelial growth factor receptor 2 antibody, anti VEGFR 2 antibody, anti VEGFR antibody, anti VEGFR-2 antibody, anti VEGFR2 antibody

Western blot analysis of anti-KDR/FLK1 Pab in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

VEGFR2 (FLK1/KDR) Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

KDR

UniProt

P35968

RefSeq (Protein):NP_002244.1