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Description
Research Area
,Product Categories/Antibodies,Loading control
Images & Validation
−Item 1 of 4
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | Western Blot 1:2000 - 1:4000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region. |
| Target | Vinculin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294416 anti-Vinculin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
All lanes: Anti-Vinculin antibody - Loading Control at 1/3000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294416 overnight at 4°C.
All lanes: Anti-Vinculin antibody - Loading Control at 1/3000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294416 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse testis labeling Vinculin with orb1294416 at 1/300.
Quick Database Links
Gene Symbol
Vinculin
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Vinculin (orb1294416)
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