von Willebrand Factor Antibody / vWF

SKU: orb1825795

Description

Human von Willebrand factor (VWF or factor VIII-related antigen) is a 309 kDa multimeric plasma glycoprotein, important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Von Willebrand factor is synthesized by endothelial cells and is reported to be expressed in a number of tumors of vascular origin. IHC results aid in the classification of acute myeloid leukemia FAB type M7, angiosarcoma and epithelioid hemangioendothelioma.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: VWF/7979R
• Immunogen: A recombinant partial protein (within amino acids 1150-1250) from the human protein was used as the immunogen for the von Willebrand Factor antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the von Willebrand Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This von Willebrand Factor antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human uterus tissue with vWF antibody (clone VWF/7979R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free vWF antibody (clone VWF/7979R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04275