von Willebrand Factor Antibody / vWF

SKU: orb385836

Description

von Willebrand Factor (vWF) is a glycoprotein produced by bone marrow cells and endothelial cells lining the inside surface of blood vessels. Its primary function is platelet adhesion, binding to Factor VIII, collagen and platelets, to coagulate blood at the site of wounding. The enzyme VWFCP, or vWF-cleaving protease, facilitates clotting by cutting the protein into subunits, increasing its binding capacity.Deficiency or dysfunction of the protein increases the tendency of wounds to bleed, or to bleed more. �Over 300 gene mutations have been identified and classified into three types. �Type 1 von Willebrand Factor disease is characterized by reduced amounts in the bloodstream, Type 2 by reduced binding ability and Type 3 by a nonfunctional protein.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Variations in protocols, secondaries and substrates may require the von Willebrand Factor antibody to be titered for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: WFA52-2
• Immunogen: A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor antibody.
• Purity: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the von Willebrand Factor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This von Willebrand Factor antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas and von Willebrand Factor antibody (clone WFA52-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC staining of human bone marrow with von Willebrand Factor antibody (clone WFA52-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

Quick Database Links

NCBI Gene ID

7450