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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Primary Antibodies;Cell Biology
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| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB29744 |
| Immunogen | Synthetic Peptide |
| Target | DCAF8L2 |
| Molecular Weight | 71191 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-DDB1-and CUL4-associated factor 8-like protein 2, DCAF8L2, WDR42C antibody
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Anti-WDR42C Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of WDR42C Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
WDR42C Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WDR42C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001130005.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
WDR42C Antibody (N-term) (orb29870)
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