Western Blot Kit

SKU: orb342271

Description

Western Blot Kit

Research Area

Product Categories/Products/ELISA Kits,Detection Kit Type: Chemiluminescent Western Blot Kit

Images & Validation

• Tested Applications: WB
• Application Notes:
  Biorbyt’ Chemiluminescent Western Blot Kit, namely our FemtoMax Chemiluminescent Substrate Kit for Western Blotting, combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol substrate. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 and is stable for at least 1 year when stored at +4°C.

Key Properties

• Reactivity: Mouse
• Conjugation: Unconjugated
• Purity: This kit contains: anti-mouse peroxidase, mouse IgG control, BSA, tween-20, and Femtomax, along with the protocol and additional supplies. See kit insert for complete details and instructions.

Storage & Handling

• Storage: See kit insert for complete instructions.
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit

Chemiluminescent Western Blot Kit shows super sensitive signal. Known amounts of GST were spiked into a HeLa lysate followed by separation by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred to a Protran B85 membrane for 1 hour at 100 mV. The membrane was blocked with 5% BLOTTO (p/n orb348624) in TTBS for 1 h at 4 °C. The blot was probed for 40 min with 1:1000 dilution of Mab anti-GST (p/n orb344400) followed by HRP anti-Mouse IgG (p/n orb347385) at 1:10000 dilution in blocking buffer for 1 h at 4°C.