WNT5A Antibody (Center)

SKU: orb35973

Description

Rabbit polyclonal antibody to WNT5A

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Wnt,Epigenetics,Developmental Biology,Primary Antibodies;Cancer;Neuroscience;Signal Transduction;Catalog Updated;Anti-Wnt-Pathway (plus Lgr5) Antibodies

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22340
• Immunogen: Synthetic Peptide
• Target: WNT5A
• Molecular Weight: 42339
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-hWNT 5A antibody, anti-hWNT5A antibody, anti-Protein Wnt 5a antibody, anti-Protein Wnt-5a antibody, anti-Protein Wnt5a antibody, anti-Wingless type MMTV integration site family member 5A antibody, anti-Wnt 5a antibody, anti-WNT 5A protein antibody, anti-WNT 5A protein precursor antibody, anti-WNT5A antibody, anti-WNT5A protein precursor antibody, anti-WNT5A_HUMAN antibody

WNT5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

WNT5A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT5A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-WNT5A Antibody (Center) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: PANC-1 whole cell lysate. Lane 3: mouse heart lysate. Lane 4: rat heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-WNT5A Antibody (Center) at 1:500 dilution. Lane 1: Hela whole cell lysate. Lane 2: mouse brain lysate. Lane 3: mouse heart lysate. Lane 4: PANC-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

WNT5A

UniProt

P41221

RefSeq (Protein):NP_003383.2, NP_001243034.1