XCT Antibody / SLC7A11

SKU: orb2635780

Description

xCT, also known as SLC7A11 (solute carrier family 7, (cationic amino acid transporter, y+ system) member 11) or CCBR1, is a 501 amino acid multi-pass membrane protein that belongs to the polyamine-organocation superfamily of amino acid transporters. Existing as a disulfide-linked heterodimer with CD98, xCT functions as a member of a heteromeric Na+-independent anionic amino acid transport system that specifically facilitates the exchange of anionic amino acids for anionic forms of cystine and glutamate, thereby mediating the formation of glutathione within the cell. Due to its involvement in amino acid transport, xCT is associated with the pathogenesis of gliomainduced neurodegeneration and brain edema, as well as pancreatic cancer. The gene encoding xCT maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant xCT antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: SLC7A11/9136R
• Immunogen: A recombinant fragment of human SLC7A11 protein was used as the immunogen for the recombinant xCT antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant xCT antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human triple negative breast cancer tissue with recombinant xCT antibody (clone SLC7A11/9136R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant xCT antibody (clone SLC7A11/9136R) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q9UPY5