YAP1 Antibody, KO Validated

SKU: orb1274561

Description

YAP1 Antibody, KO Validated

Research Area

,Cancer,Cell Cycle,Signal Transduction,Transcription

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
• Target: YAP1
• Molecular Weight: Observed: 72kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

YAP1, YAP, YAP2, YAP65, YKI

Western blot analysis of extracts of various cell lines, using YAP1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using YAP1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

YAP1

UniProt

P46937