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Description
Research Area
,Ovarian Cancer
Images & Validation
−
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate));,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | PCRP-YBX3-2D12 |
| Immunogen | Recombinant full-length human YBX3 protein |
| Target | YBX3 |
| Source/Expression System | CHO cells |
| Molecular Weight | 40.09kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Y-Box Binding Protein; ZONAB; CSDA1; CSDA; Single-Strand DNA-Binding Protein NF-GMB; Cold Shock Domain-Containing Protein A; Cold-Shock Domain Containing A1; Y-Box-Binding Protein; DNA-Binding Protein; DbpA; DBPA; ZO-1-Associated Nucleic Acid-Binding Protein; Cold Shock Domain Protein A; Cold-Shock Domain Protein A
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
YBX3 (Transcription Factor) Antibody (orb2311125)
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