YY1 Mouse Monoclonal Antibody (Nuclear Loading Control)

SKU: orb783416

Description

YY1 Mouse Monoclonal Antibody (Nuclear Loading Control)

Research Area

Neurogenesis, Neuroscience, Transcription Factors, DNA and RNA Research, Zinc Finger Protein, DNA and RNA Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:25-50, IHC-F=1:25-50, IF=1:100-500
• Reactivity: Fish, Human, Monkey, Rat, Zebrafish
• Predicted Reactivity: Fish, Monkey, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: G23F3
• Immunogen: Recombinant human YY1 (His-tagged).
• Target: YY1
• Molecular Weight: 46 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Yin Yang 1; YY1 transcription factor; CF1; delta; delta transcription factor; NF E1; NFE1; Transcriptional repressor protein YY1; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin Yang 1; Ying Yang 1; YY 1; YY 1 transcription factor; TYY1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Monkey brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (orb783416) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (orb783416) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Zebra fish brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (orb783416) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: 293 cell lysates, Lane 2: C6 cell lysates, Lane 3: Hela cell lysates, Lane 4: U-251MG cell lysates, Primary: Anti-YY1 (orb783416) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 65 kD.

Sample: Lane 1: Daudi cell lysates, Lane 2: U251 cell lysates, Primary: Anti-YY1 (orb783416) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 68 kD.

Quick Database Links

Gene Symbol

YY1

UniProt

P25490