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Description
Research Area
,Apoptosis|Autophagy|Cardiovascular|Developmental Biology|Nuclear Marker|Signal Transduction|Transcription Factors
Images & Validation
−
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2 |
| Clone No. | YY1/5436 |
| Immunogen | His-tagged YY1 protein |
| Target | YY1 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 40/68kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab with BSA and Azide at 200ug/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CF1; Delta transcription factor; INO80 complex subunit S; NF E1; NFE1; Transcriptional repressor protein YY1; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin and Yang 1 protein; YY-1; YY1 transcription factor
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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