ZNF276 Antibody

SKU: orb2634791

Description

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF276 is a 614 amino acid protein containing five C2H2- type zinc fingers and one zinc finger associated (ZAD) domain. Due to a loss of heterozygosity at the chromosomal location of the gene encoding ZNF276 in sporadic breast cancers, the ZNF276 gene has been targeted as a possible breast cancer tumor suppressor. The FANCA gene, which encodes a DNA repair protein, is situated at the same chromosomal location as the ZNF276 gene, suggesting a possible involvement of ZNF276 in the progression of Fanconi anemia, an autosomal recessive disorder which is caused by mutations in the gene encoding FANCA. There are two isoforms of ZNF276 that exist as a result of an alternative splicing event.

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the ZNF276 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: PCRP-ZNF276-1A5
• Immunogen: Recombinant full-length human ZNF276 protein was used as the immunogen for the ZNF276 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This ZNF276 antibody is available for research use only.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZNF276 antibody (clone PCRP-ZNF276-1A5). These results demonstrate the foremost specificity of the PCRP-ZNF276-1A5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF276 antibody (green, clone PCRP-ZNF276-1A5) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with ZNF276 antibody (blue, clone PCRP-ZNF276-1A5), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free ZNF276 antibody (clone PCRP-ZNF276-1A5) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q8N554