Anti-Alpha-synuclein (Phospho-S129) Antibody

SKU: orb304600

Description

Rabbit polyclonal antibody to alpha synuclein (phospho-S129)

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Signaling Pathways/Alzheimer's

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Primate, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S129 of human Alpha-synuclein protein. The exact sequence is proprietary.
• Target: SNCA
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-alpha synuclein antibody, anti- Alpha-synuclein antibody, anti- Alpha-synuclein, isoform NACP140 antibody, anti- alphaSYN antibody, anti- MGC105443 antibody, anti- MGC110988 antibody, anti- MGC127560 antibody, anti- MGC64356 antibody, anti- NACP antibody, anti- Non A beta component of AD amyloid antibody, anti- Non A4 component of amyloid antibody, anti- Non A4 component of amyloid precursor antibody, anti- Non-A beta component of AD amyloid antibody, anti- Non-A-beta component of alzheimers disease amyloid, precursor of antibody, anti- Non-A4 component of amyloid precursor antibody, anti- Non-A4 component of amyloid, precursor of antibody, anti- OTTHUMP00000218549 antibody, anti- OTTHUMP00000218551 antibody, anti- OTTHUMP00000218552 antibody, anti- OTTHUMP00000218553 antibody, anti- OTTHUMP00000218554 antibody, anti- PARK 1 antibody, anti- PARK 4 antibody, anti- PARK1 antibody, anti- PARK4 antibody, anti- Parkinson disease (autosomal dominant, Lewy body) 4 antibody, anti- Parkinson disease familial 1 antibody, anti- PD 1 antibody, anti- PD1 antibody, anti- SNCA antibody, anti- Snca synuclein, alpha (non A4 component of amyloid precursor) antibody, anti- SYN antibody, anti- Synuclein alpha antibody, anti- Synuclein alpha 140 antibody, anti- Synuclein, alpha (non A4 component of amyloid precursor) antibody, anti- SYUA_HUMAN antibody

Western blot analysis of Alpha-synuclein (Phospho-S129) expression in HEK293T (A), mouse brain (B) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 18 kD)

Immunohistochemical analysis of Alpha-synuclein (Phospho-S129) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Alpha-synuclein (Phospho-S129) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

SNCA

UniProt

P37377, O55042, P37840

NCBI Gene ID

29219, 6622, 20617