Anti-Apolipoprotein E Antibody

SKU: orb213571

Description

Rabbit polyclonal antibody to Apolipoprotein E

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Signaling Pathways/Alzheimer's

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Reactivity: Human, Mouse, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Apolipoprotein E. The exact sequence is proprietary.
• Target: APOE
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-AD2 antibody, Anti-Apo-E antibody, Anti-APOE antibody, Anti-APOE_HUMAN antibody, Anti-APOEA antibody, Anti-Apolipoprotein E antibody, Anti-Apolipoprotein E3 antibody, Anti-ApolipoproteinE antibody, Anti-Apoprotein antibody, Anti-LDLCQ5 antibody, Anti-LPG antibody

Western blot analysis of Apolipoprotein E expression in A375 (A), U2OS (B), DLD (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)

Immunohistochemical analysis of Apolipoprotein E staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Apolipoprotein E staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

APOE

UniProt

P08226, P02650, P02649

NCBI Gene ID

348, 11816, 25728