Anti-ATF6 Antibody

SKU: orb340705

Description

Rabbit polyclonal antibody to ATF6

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide of human ATF6
• Target: ATF6
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Activating transcription factor 6 alpha antibody, anti-Activating transcription factor 6 antibody, anti-ATF 6 antibody, anti-ATF6 alpha antibody, anti-ATF6 antibody, anti-ATF6-alpha antibody, anti-ATF6A antibody, anti-cAMP dependent transcription factor ATF 6 alpha antibody, anti-ESTM49 antibody, anti-FLJ21663 antibody, anti-Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody

Western blot analysis of ATF6 expression in K562 (A), PC3 (B), mouse heart (C) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 90-100 kD)

Immunohistochemical analysis of ATF6 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ATF6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

ATF6

UniProt

P18850, G3V909, F6VAN0

NCBI Gene ID

226641, 304962, 22926