Anti-CD274 Antibody

SKU: orb705620

Description

Rabbit polyclonal antibody to CD274

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human CD274. The exact sequence is proprietary.
• Target: CD274
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-B7H1 antibody, anti-PDCD1L1 antibody, anti-PDCD1LG1 antibody, anti-PDL1 antibody, anti-Programmed cell death 1 ligand 1 antibody, anti-PD-L1 antibody, anti-PDCD1 ligand 1 antibody, anti-Programmed death ligand 1 antibody, anti-B7 homolog 1 antibody, anti-B7-H1 antibody, anti-CD274 antibody

Western blot analysis of CD274 expression in A549 (A) whole cell lysates. (Predicted band size: 20; 33 kD; Observed band size: 40-50 kD)

Immunohistochemical analysis of CD274 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD274 staining in human breast cancer. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

CD274

UniProt

Q9EP73, Q9NZQ7

NCBI Gene ID

60533, 29126