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Description
Images & Validation
−| Tested Applications | ELISA, FA, FC, ICC, IHC-Fr, IP |
|---|---|
| Dilution Range | Functional application: The antibody W6/32 is suitable as a positive control for HLA tissue typing. Flow cytometry: Recommended dilution: 1-4 µg/ml |
| Reactivity | Bovine, Feline, Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | W6/32 |
| Immunogen | Membrane of human tonsil cells |
| Target | HLA Class I |
| Purity | Purified by protein-A affinity chromatography. |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Separation of leukocytes stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 μg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 μg/ml, GAM APC).
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Anti-HLA Class I Purified Low Endotoxin (orb179914)
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