Anti-HSP27 Antibody

SKU: orb1474330

Description

Mouse monoclonal antibody to HSP27

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Reactivity: Human, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human Hsp27 protein fragments expressed in E.coli.
• Target: HSPB1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Western blot analysis of HSP27 expression in Hela (A), Hela (B), A431 (C), COS7 (D), Lncap (E), MCF7 (F) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 27 kD)

Immunofluorescent analysis of HSP27 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792

NCBI Gene ID

3315