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Anti-Hu CD45 Purified

SKU: orb44266

Description

Mouse monoclonal antibody to CD45

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

Tested ApplicationsFC, ICC, IHC-P, IP, WB
Dilution RangeImmunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1 μg/ml.
ReactivityHuman
Application Notes
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa). Flow cytometry: Recommended dilution: 1 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.MEM-28
ImmunogenHuman thymocytes and T lymphocytes.
TargetCD45
PurityPurified by protein-A affinity chromatography.
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti CD 45 antibody, anti CD45 antibody, anti CD45 antigen antibody, anti CD45R antibody, anti L CA antibody, anti L-CA antibody, anti LCA antibody, anti Leukocyte common antigen antibody, anti loc antibody, anti Ly-5 antibody, anti LY5 antibody, anti Ly5, homolog of antibody, anti Lyt-4 antibody, anti Protein tyrosine phosphatase receptor type C antibody, anti Protein tyrosine phosphatase receptor type c polypeptide antibody, anti protein tyrosine phosphatase, receptor type, C antibody, anti PTPRC antibody, anti Receptor-type tyrosine-protein phosphatase C antibody, anti SCID due to PTPRC deficiency antibody, anti T200 antibody, anti T200 glycoprotein antibody, anti T200 leukocyte common antigen antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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0.1 mg
$ 280.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days.
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