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Description
Research Area
Epigenetics, Infectious Diseases, Neuroscience
Images & Validation
−| Tested Applications | FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1 μg/ml. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-28 |
| Immunogen | Human thymocytes and T lymphocytes. |
| Target | CD45 |
| Purity | Purified by protein-A affinity chromatography. |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti CD 45 antibody, anti CD45 antibody, anti CD45 antigen antibody, anti CD45R antibody, anti L CA antibody, anti L-CA antibody, anti LCA antibody, anti Leukocyte common antigen antibody, anti loc antibody, anti Ly-5 antibody, anti LY5 antibody, anti Ly5, homolog of antibody, anti Lyt-4 antibody, anti Protein tyrosine phosphatase receptor type C antibody, anti Protein tyrosine phosphatase receptor type c polypeptide antibody, anti protein tyrosine phosphatase, receptor type, C antibody, anti PTPRC antibody, anti Receptor-type tyrosine-protein phosphatase C antibody, anti SCID due to PTPRC deficiency antibody, anti T200 antibody, anti T200 glycoprotein antibody, anti T200 leukocyte common antigen antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation








