Anti-Hu CD45 Purified

SKU: orb44725

Description

Mouse monoclonal antibody to CD45

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Dilution range: Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Reactivity: Human, Primate
• Application Notes:
  Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: HI30
• Immunogen: human peripheral blood leukocytes
• Target: CD45
• Purity: Purified by protein-G affinity chromatography.
• Purification: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

anti CD 45 antibody, anti CD45 antibody, anti CD45 antigen antibody, anti CD45R antibody, anti L CA antibody, anti L-CA antibody, anti LCA antibody, anti Leukocyte common antigen antibody, anti loc antibody, anti Ly-5 antibody, anti LY5 antibody, anti Ly5, homolog of antibody, anti Lyt-4 antibody, anti Protein tyrosine phosphatase receptor type C antibody, anti Protein tyrosine phosphatase receptor type c polypeptide antibody, anti protein tyrosine phosphatase, receptor type, C antibody, anti PTPRC antibody, anti Receptor-type tyrosine-protein phosphatase C antibody, anti SCID due to PTPRC deficiency antibody, anti T200 antibody, anti T200 glycoprotein antibody, anti T200 leukocyte common antigen antibody

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (HI30) purified, GAM-APC.

Anti-Hu CD45 Purified (clone HI30) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI30 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.

Quick Database Links

Gene Symbol

CD45

UniProt

P08575

NCBI Gene ID

5788