Oct4/POU5F1 Antibody

SKU: orb865716

Description

Anti-Oct4/POU5F1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Oct4/POU5F1, which shares 94.1% amino acid (aa) sequence identity with mouse POU5F1.
• Molecular Weight: 39-45 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3

Western blot analysis of Oct4/POU5F1 using anti-Oct4/POU5F1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oct4/POU5F1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Oct4/POU5F1 at approximately 39-45 kDa. The expected band size for Oct4/POU5F1 is at 39 kDa.

Quick Database Links

UniProt

Q01860