Anti-p53 Purified

SKU: orb43682

Description

Mouse monoclonal antibody to p53

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml, overnight at 4°C; positive control: RAMOS human lymphoma cell line, non-reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: BP53-12
• Immunogen: Bacterially expressed full-length wild-type p53
• Target: p53
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BCC7 antibody, anti FLJ92943 antibody, anti HGNC11998 antibody, anti LFS1 antibody, anti p53 antibody, anti p53 Cellular Tumor Antigen antibody, anti p53 tumor suppressor antibody, anti Phosphoprotein p53 antibody, anti Tp53 antibody, anti TRP53 antibody, anti Tumor protein 53 antibody, anti Tumor protein p53 antibody, anti TP53 antibody

Quick Database Links

Gene Symbol

p53

UniProt

P04637

NCBI Gene ID

7157