Anti-PLK1 Antibody

SKU: orb1422481

Description

Rabbit polyclonal antibody to PLK1.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
• Reactivity: Human, Mouse, Porcine, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: PLK1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Immunofluorescent analysis of PLK1 staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of PLK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of PLK1 expression in zebrafish (A) whole cell lysates.

Quick Database Links

UniProt

P53350