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Description
Images & Validation
−Item 1 of 3
| Tested Applications | IEM, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500 |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Antigen: Purified recombinant RFP peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
| Target | Red Fluorescent Protein |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
5 ResultsDilution
Sample
Summary
IF
Mouse
1:10,000
Brain sections from adult mouse
Sample preparation involved transcardial perfusion with...
IF
Rat
1:200
Cultured cells and to longitudinal sections of rat sciatic nerve tissue
For cultured cells, fixation was performed...
WB
Mouse
1:1000
Mouse hippocampal tissue
Sample preparation involved homogenizing hippocampal tissue...
ICC
Mouse
1:200
Free-floating sagittal mouse brain sections
The antibody was applied to free-floating...
IF
Rat
1:200
Grafted Schwann cells (SCs)
Sample preparation involved grafting either repair...
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunohistochemical analysis of formalin-fixed and paraffin-embedded epithelium cells using RFP antibody
Western blot analysis of staining of WB293HEK cells transduced with tdTomato Ad lysates using RFP antibody
Immunofluorescence analysis of 293HEK cells using RFP antibody
Quick Database Links
Gene Symbol
Red Fluorescent Protein
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
anti-RFP (orb334992)
- 0.0
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