Beta Tubulin Antibody Loading Control

SKU: orb248111

Description

There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 2-4ug/ml
• Reactivity: Canine, Human, Mouse, Primate, Rat
• Application Notes:
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody.
• Purity: Protein A Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This beta Tubulin antibody loading control is available for research use only.
• Disclaimer: For research use only

Quick Database Links

NCBI Gene ID

203068