BTRC Antibody (N-term)

SKU: orb134924

Description

Rabbit polyclonal to BRTC1/2. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at Lys-21 and Lys-22. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Wnt,Infectious Diseases,Crown Antibodies;Primary Antibodies;Antibody Collections;Cell Biology;Microbiology;Neuroscience;Anti-Wnt-Pathway (plus Lgr5) Antibodies;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Target: BTRC
• Molecular Weight: 68867
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BTRC antibody, anti BTRCP antibody, anti FBW1A antibody, anti FBXW1A antibody, anti F-box/WD repeat-containing protein 1A antibody, anti F-box/WD repeat-containing protein 1A antibody, anti E3RSIkappaB antibody, anti F-box/WD repeat-containing protein 1A antibody, anti Epididymis tissue protein Li 2a antibody, anti F-box/WD repeat-containing protein 1A antibody, anti F-box and WD repeats protein beta-TrCP antibody, anti F-box/WD repeat-containing protein 1A antibody, anti pIkappaBalpha-E3 receptor subunit antibody

Anti-BTRC Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

BTRC

UniProt

Q9Y297

RefSeq (Protein):NP_003930.1, NP_378663.1