BTRC Antibody (N-term)

SKU: orb652195

Description

Rabbit polyclonal antibody to BTRC

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Wnt,Infectious Diseases,Primary Antibodies;Cell Biology;Microbiology;Neuroscience;Anti-Wnt-Pathway (plus Lgr5) Antibodies;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB16275
• Immunogen: Synthetic Peptide
• Target: BTRC
• Molecular Weight: 68867
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-F-box/WD repeat-containing protein 1A antibody, anti-E3RSIkappaB antibody, anti-Epididymis tissue protein Li 2a antibody, anti-F-box and WD repeats protein beta-TrCP antibody, anti-pIkappaBalpha-E3 receptor subunit antibody, anti-BTRC antibody, anti-BTRCP antibody, anti-FBW1A antibody, anti-FBXW1A antibody

BTRC Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the BTRC antibody detected the BTRC protein (arrow).

BTRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-BTRC Antibody (N-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: 293 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of BTRC Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

BTRC

UniProt

Q9Y297

RefSeq (Protein):NP_003930.1, NP_378663.1