CCAR2 Antibody

SKU: orb1256573

Description

CCAR2 Antibody

Research Area

,Apoptosis,Cancer,Cell Cycle

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3).
• Target: CCAR2
• Molecular Weight: Observed: 100kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein

Western blot analysis of extracts of various cell lines, using CCAR2 antibody (orb1256573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using CCAR2 antibody (orb1256573). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CCAR2

UniProt

Q8N163